Hi, I'm trying to culture L. pneumophila in liquid culture using ACES buffered yeast extract broth (10g/L yeast extract, 0.4g/L L-cystein, 0.25g/L ferric pyrophosphate). Medium is filter sterilized.
Charcoal Yeast Extract (BCYE) Agar.4 In 1981, Edelstein added α-ketoglutarate to BCYE Agar which improved the recovery of Legionella pneumophila from contaminated environmental samples. 5 Dennis et al. recommended the use of BCYE w/ GPCV (i.e., glycine, Yeast Extract provides sources of nitrogen, carbon, and vitamins in BCYE Agar. Activated Charcoal decomposes hydrogen peroxide and other toxic products. ACES Buffer was added to formulation to get a stable pH for optimal growth. α-Ketoglutarate stimulates organism growth. Agar is the solidifying agent. L-Cysteine, in the supplement, is an Allow sample to cool to room temperature before reading pH. Keep charcoal in suspension when dispensing. For solid medium, add 17 grams of Agar to base medium above. **Note: Medium should be wrapped in foil to protect from light. **Note: Be sure to allow solid products adequate time to dry (at least two days). Agar Hardy Diagnostics Buffered Charcoal Yeast Extract (BCYE) Agar formulations are recommended for use in the cultivation and primary isolation of Legionella spp. in water and other samples suspected of harboring the bacteria. Do not ingest, inhale, or allow to come into contact with skin. This product is for in vitro diagnostic use only. In the experimental study, all 10 clinical isolates produced trails on buffered charcoal-yeast extract agar, and the mean recovery after 10 days of incubation ranged from 38% to 95% of the original inoculum number. For seven of the 10 isolates, more than 70% of the original inoculum was recovered on buffered charcoal-yeast extract agar. Thermo Scientific Remel Non-supplemented Buffered CYE Agar (Buffered charcoal yeast extract-BCYE) is used for the presumptive identification of Legionella spp. by satellite phenomena. The medium is identical to Buffered Charcoal Yeast Extract (BCYE) agar except for the addition of the four selective compounds. Yeast extract provides sources of nitrogen, carbon and vitamins. Activated charcoal neutralises toxic metabolic by-products and ACES and potassium hydroxide buffer the media to the optimum pH.
Buffered Charcoal Yeast Extract (BCYE) Feeley et al in 1978 developed a medium to isolate Legionella species which was later modified by substituting yeast extract for casein hydrolysate and beef extract, and replacing starch with activated charcoal and named it as Charcoal Yeast Extract (CYE) Agar.
BD BBL™ BCYE (Buffered Charcoal Yeast Extract) Agar is used for the isolation and cultivation of Legionella species. II PERFORMANCE TEST PROCEDURE 1. Inoculate representative samples with the cultures listed below. a. Add 0.1 mL of a culture containing 30–300 CFU/0.1 mL to each plate and spread-inoculate using a sterile glass spreader. b. Mixed culture on buffered charcoal yeast extract agar 5 days at 35C, 0.63X. Suspect Nocardia colonies are visible as round, white fuzzy colonies each surrounded by a halo of substrate mycelium. 3 of 4 Charcoal Yeast Extract (CYE) Agar was developed by Feely et al. in 1979 as a modification of an existing medium, F-G Agar.1,2 They replaced the starch in the F-G agar with activated charcoal and substituted yeast extract for casein hydrolysate, resulting in better recovery of L. pneumophila.
In the experimental study, all 10 clinical isolates produced trails on buffered charcoal-yeast extract agar, and the mean recovery after 10 days of incubation ranged from 38% to 95% of the original inoculum number. For seven of the 10 isolates, more than 70% of the original inoculum was recovered on buffered charcoal-yeast extract agar.
Charcoal Yeast Extract (CYE) Agar was developed by Feely et al. in 1979 as a modification of an existing medium, F-G Agar.1,2 They replaced the starch in the F-G agar with activated charcoal and substituted yeast extract for casein hydrolysate, resulting in better recovery of L. pneumophila. Hi, I'm trying to culture L. pneumophila in liquid culture using ACES buffered yeast extract broth (10g/L yeast extract, 0.4g/L L-cystein, 0.25g/L ferric pyrophosphate). Medium is filter sterilized. The plates, upon spread of lysed blood sediment from the Isolator tube, were incubated for 5 days with 5% carbon dioxide. Numerous colonies grew on buffered charcoal yeast extract agar (top right), fewer and smaller colonies on the chocolate agar (top left and lower right), and none on blood agar (lower left). Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain bacteria, particularly the Gram-negative species Francisella tularensis, Legionella pneumophila and Haemophilus influenzae. [citation needed] It has also been used for the recovery of Acanthamoeba keratitis. References